SLC7A5 Knockout cell line (Hep G2)

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SLC7A5 Knockout cell line (Hep G2) Order

SLC7A5 Knockout cell line (Hep G2)
Catalog#
YKO-HT1915
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
SLC7A5 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
SLC7A5 Knockout cell line (Hep G2)
Species
Human
Gene Name
SLC7A5
Gene ID
8140
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 1 (based on transcript SLC7A5-201)
Validation: PCR+Sanger Sequencing
SLC7A5 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the SLC7A5 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, SLC7A5 gene knockout SLC7A5 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
SLC7A5 Knockout cell line (Hep G2)
SLC7A5 Knockout cell line (Hep G2)
Catalog#
YKO-HT1915
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

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Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • SLC7A5 Knockout cell line (THP-1)

    SLC7A5 Knockout cell line (THP-1)

    Organism: Human
    Gene Name: SLC7A5
    Gene ID: 8140
    Size: 1*10^6
    Catalog#: YKO-HT0432
    YKO-HT0432(THP-1)
  • SLC7A5 Knockout cell line (HeLa)

    SLC7A5 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: SLC7A5
    Gene ID: 8140
    Size: 1*10^6
    Catalog#: YKO-H759
    YKO-H759(HeLa)
  • SLC7A5 Knockout cell line (HCT 116)

    SLC7A5 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: SLC7A5
    Gene ID: 8140
    Size: 1*10^6
    Catalog#: YKO-HS12630
    YKO-HS12630(HCT 116)

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Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium

KO Cell Lines

  • SLC7A5 Knockout cell line (THP-1)

    SLC7A5 Knockout cell line (THP-1)

    Organism: Human
    Gene Name: SLC7A5
    Gene ID: 8140
    Size: 1*10^6
    Catalog#: YKO-HT0432
  • SLC7A5 Knockout cell line (HeLa)

    SLC7A5 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: SLC7A5
    Gene ID: 8140
    Size: 1*10^6
    Catalog#: YKO-H759
  • SLC7A5 Knockout cell line (HCT 116)

    SLC7A5 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: SLC7A5
    Gene ID: 8140
    Size: 1*10^6
    Catalog#: YKO-HS12630

SLC7A5 Knockout cell line (Hep G2) Order

SLC7A5 Knockout cell line (Hep G2)
Catalog#
YKO-HT1915
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
SLC7A5 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
SLC7A5 Knockout cell line (Hep G2)
Species
Human
Gene Name
SLC7A5
Gene ID
8140
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 1 (based on transcript SLC7A5-201)
Validation: PCR+Sanger Sequencing
SLC7A5 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the SLC7A5 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, SLC7A5 gene knockout SLC7A5 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
SLC7A5 Knockout cell line (Hep G2)
SLC7A5 Knockout cell line (Hep G2)
Catalog#
YKO-HT1915
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • SLC7A5 Knockout cell line (THP-1)

    SLC7A5 Knockout cell line (THP-1)

    Organism: Human
    Gene Name: SLC7A5
    Gene ID: 8140
    Size: 1*10^6
    Catalog#: YKO-HT0432
    YKO-HT0432(THP-1)
  • SLC7A5 Knockout cell line (HeLa)

    SLC7A5 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: SLC7A5
    Gene ID: 8140
    Size: 1*10^6
    Catalog#: YKO-H759
    YKO-H759(HeLa)
  • SLC7A5 Knockout cell line (HCT 116)

    SLC7A5 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: SLC7A5
    Gene ID: 8140
    Size: 1*10^6
    Catalog#: YKO-HS12630
    YKO-HS12630(HCT 116)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium

KO Cell Lines

  • SLC7A5 Knockout cell line (THP-1)

    SLC7A5 Knockout cell line (THP-1)

    Organism: Human
    Gene Name: SLC7A5
    Gene ID: 8140
    Size: 1*10^6
    Catalog#: YKO-HT0432
  • SLC7A5 Knockout cell line (HeLa)

    SLC7A5 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: SLC7A5
    Gene ID: 8140
    Size: 1*10^6
    Catalog#: YKO-H759
  • SLC7A5 Knockout cell line (HCT 116)

    SLC7A5 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: SLC7A5
    Gene ID: 8140
    Size: 1*10^6
    Catalog#: YKO-HS12630

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